Proteintech, 82828-14-RR, Acetyl-Histone H3 (Lys36) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H3 (Lys36) (82828-14-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 82828-14-RR targets Acetyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Trichostatin A treated NIH/3T3 cells Positive IF/ICC detected in: Trichostatin A treated NIH/3T3 cells Positive Dot Blot detected in: N/A N/A Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information H3K36ac peaks at the 5' end of genes,manily on the two nucleosomeus immediately distal to the transcription start site, independently of gene length. H3K36ac and H3K36mac showed negative creastalk, which is mediated by GCN3 and the histone methyl transferase SDX8. Although H3K36ac is associated with gene activity, there is no linear relationship between H3K36ac and transcript levels, suggesting that H3K36ac is a binary indicator of transcription. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Observed Molecular Weight: 15 kDa GenBank Accession Number: BC066245 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.