Product Description
Size: 20ul / 100ul
The BACH1 (82842-7-RR) by Proteintech is a Recombinant antibody targeting BACH1 in WB, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, rat, pig samples
82842-7-RR targets BACH1 in WB, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, rat, pig samples.
Tested Applications
Positive WB detected in: HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, rat brain tissue, pig brain tissue
Positive IF/ICC detected in: HeLa cells
Positive ChIP-qPCR detected in: K-562 cells
Recommended dilution
Western Blot (WB): WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
CHIP-QPCR: CHIP-QPCR : 1:10-1:100
Background Information
BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.
Specification
Tested Reactivity: human, rat, pig
Host / Isotype: Rabbit / IgG
Class: Recombinant
Type: Antibody
Immunogen: CatNo: Ag5128 Product name: Recombinant human BACH1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 387-736 aa of BC063307 Sequence: SSVEREVAEHLAKGFWSDICSTDTPCQMQLSPAVAKDGSEQISQKRSECPWLGIRISESPEPGQRTFTTLSSVNCPFISTLSTEGCSSNLEIGNDDYVSEPQQEPCPYACVISLGDDSETDTEGDSESCSAREQECEVKLPFNAQRIISLSRNDFQSLLKMHKLTPEQLDCIHDIRRRSKNRIAAQRCRKRKLDCIQNLESEIEKLQSEKESLLKERDHILSTLGETKQNLTGLCQKVCKEAALSQEQIQILAKYSAADCPLSFLISEKDKSTPDGELALPSIFSLSDRPPAVLPPCARGNSEPGYARGQESQQMSTATSEQAGPAEQCRQSGGISDFCQQMTDKCTTDE Predict reactive species
Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
Calculated Molecular Weight: 82 kDa
Observed Molecular Weight: 100-110 kDa
GenBank Accession Number: BC063307
Gene Symbol: BACH1
Gene ID (NCBI): 571
ENSEMBL Gene ID: ENSG00000156273
RRID: AB_3086551
Conjugate: Unconjugated
Form: Liquid
Purification Method: Protein A purification
UNIPROT ID: O14867
Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.
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Collaboration
Tony Tang
Email: Tony.Tang@iright.com
Mobile/WhatsApp/Wechat: +86-17717886924