Proteintech, 82876-1-RR, SAGE1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SAGE1 (82876-1-RR) by Proteintech is a Recombinant antibody targeting SAGE1 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82876-1-RR targets SAGE1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: MDA-MB-453 cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Sarcoma antigen 1 (SAGE1) is a cancer/testis antigen that is limited to the spermatogonia and pre-meiotic spermatocytes and localized to the nuclei (PMID: 34465596). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34216 Product name: Recombinant human SAGE1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 247-387 aa of NM_018666.2 Sequence: ATITYNVPEEKMEKGQPQPDNILSTASTGLINVAGAGTPAISTNGLYSTVPHNVCEEKMENDQPQPNNVLSTVQPVIIYLTATGIPGMNTRDQYATITHNVCEERVVNNQPLPSNALSTVLPGLAYLATADMPAMSTRDQH Predict reactive species Full Name: sarcoma antigen 1 Calculated Molecular Weight: 99kd GenBank Accession Number: NM_018666.2 Gene Symbol: SAGE1 Gene ID (NCBI): 55511 RRID: AB_3670595 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9NXZ1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.