Proteintech, 82901-1-RR, LAT Recombinant monoclonal antibody

Size: 20ul / 100ul The LAT (82901-1-RR) by Proteintech is a Recombinant antibody targeting LAT in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 82901-1-RR targets LAT in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, EL-4 cells, human platelets Positive IF/ICC detected in: Jurkat cells Positive FC (Intra) detected in: Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:5000 Immunofluorescence (IF)/ICC: IF/ICC : 1:750-1:3000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information LAT is a membrane-localized adaptor protein which is primarily concentrated in microdomains by palmitoylation. LAT plays a central role in T-cell activation by nucleating signaling complexes that are critical for the propagation of T-cell signals from the plasma membrane to the cellular interior. In addition to being a positive regulator of T cell signaling, LAT also recruits several negative regulatory proteins, including kinases, phosphatases, and ubiquitin ligases, which ultimately leads to signal termination. LAT is subject to ubiquitylation, and ubiquitin-resistant mutants of LAT display enhanced signaling. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: linker for activation of T cells Calculated Molecular Weight: 262 aa, 28 kDa Observed Molecular Weight: 36-38 kDa GenBank Accession Number: BC011563 Gene Symbol: LAT Gene ID (NCBI): 27040 RRID: AB_3670630 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O43561 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.