Proteintech, 82927-1-RR, CYP7B1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CYP7B1 (82927-1-RR) by Proteintech is a Recombinant antibody targeting CYP7B1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82927-1-RR targets CYP7B1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HepG2 cells, HeLa cells, HCT 116 cells Positive IF/ICC detected in: HepG2 cells, HEK-293 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag19374 Product name: Recombinant human CYP7B1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 96-403 aa of BC136574 Sequence: PFQYQLVIKNHKQLSFRVFSNKLLEKAFSISQLQKNHDMNDELHLCYQFLQGKSLDILLESMMQNLKQVFEPQLLKTTSWDTAELYPFCSSIIFEITFTTIYGKVIVCDNNKFISELRDDFLKFDDKFAYLVSNIPIELLGNVKSIREKIIKCFSSEKLAKMQGWSEVFQSRQDVLEKYYVHEDLEIGAHHLGFLWASVANTIPTMFWAMYYLLRHPEAMAAVRDEIDRLLQSTGQKKGSGFPIHLTREQLDSLICLESSIFEALRLSSYSTTIRFVEEDLTLSSETGDYCVRKGDLVAIFPPVLHGD Predict reactive species Full Name: cytochrome P450, family 7, subfamily B, polypeptide 1 Calculated Molecular Weight: 506 aa, 58 kDa Observed Molecular Weight: 50-55 kDa GenBank Accession Number: BC136574 Gene Symbol: CYP7B1 Gene ID (NCBI): 9420 RRID: AB_3670665 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75881 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.