Proteintech, 82980-1-RR, POU4F3 Recombinant monoclonal antibody

Size: 20ul / 100ul The POU4F3 (82980-1-RR) by Proteintech is a Recombinant antibody targeting POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 82980-1-RR targets POU4F3 in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Neuro-2a cells, mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue Positive IF/ICC detected in: U2OS cells Positive FC (Intra) detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:12000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)-P: IF-P : 1:175-1:700 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag16004 Product name: Recombinant human POU4F3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 99-184 aa of BC104923 Sequence: HPAALTSHPHHAVHQGLEGDLLEHISPTLSVSGLGAPEHSVMPAQIHPHHLGAMGHLHQAMGMSHPHTVAPHSAMPACLSDVESDP Predict reactive species Full Name: POU class 4 homeobox 3 Calculated Molecular Weight: 338 aa, 37 kDa Observed Molecular Weight: 33-40 kDa GenBank Accession Number: BC104923 Gene Symbol: POU4F3 Gene ID (NCBI): 5459 RRID: AB_3670729 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15319 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.