Proteintech, 82993-6-RR, Endoglin/CD105 Recombinant monoclonal antibody

Size: 20ul / 100ul The Endoglin/CD105 (82993-6-RR) by Proteintech is a Recombinant antibody targeting Endoglin/CD105 in WB, IHC, ELISA applications with reactivity to human samples 82993-6-RR targets Endoglin/CD105 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HUVEC cells, JAR cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information Endoglin (ENG, CD105) is a homodimeric cell membrane glycoprotein of 180 kDa, composed of disulphide-linked subunits of 90-95 kDa. Endoglin is a proliferation-associated and hypoxia-inducible protein mainly expressed on vascular endothelial cells. It acts as an accessory receptor for transforming growth factor beta (TFG-β) and is involved in vascular development and remodelling. The important role of Endoglin in angiogenesis and in tumor progression makes it an ideal target for antiangiogenic therapy and a good marker for tumor prognosis. (PMID: 1326540; 14528280; 21737653; 12773481) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: endoglin Calculated Molecular Weight: 71 kDa Observed Molecular Weight: 75-90 kDa GenBank Accession Number: BC014271 Gene Symbol: CD105 Gene ID (NCBI): 2022 RRID: AB_3670742 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P17813 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.