Proteintech, 83053-4-RR, CD61 / Integrin beta 3 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD61 / Integrin beta 3 (83053-4-RR) by Proteintech is a Recombinant antibody targeting CD61 / Integrin beta 3 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83053-4-RR targets CD61 / Integrin beta 3 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: HUVEC cells Positive FC (Intra) detected in: HUVEC cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) Calculated Molecular Weight: 87 kDa Observed Molecular Weight: 100-110 kDa GenBank Accession Number: BC127667 Gene Symbol: Integrin beta 3 Gene ID (NCBI): 3690 RRID: AB_3670778 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05106 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.