Proteintech, 83074-2-RR, PFAS Recombinant monoclonal antibody

Size: 20ul / 100ul The PFAS (83074-2-RR) by Proteintech is a Recombinant antibody targeting PFAS in WB, ELISA applications with reactivity to human samples 83074-2-RR targets PFAS in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, Raji cells, HEK-293T cells, U-251 cells Positive FC (Intra) detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.4 ug per 10^6 cells in a 100 µl suspension Background Information Phosphoribosylformylglycinamidine synthase (PFAS) is a highly conserved enzyme that catalyzes the fourth step of de novo purine synthesis. It catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag20162 Product name: Recombinant human PFAS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 989-1338 aa of BC167158 Sequence: MVRVSVNGAVVLEEPVGELRALWEETSFQLDRLQAEPRCVAEEERGLRERMGPSYCLPPTFPKASVPREPGGPSPRVAILREEGSNGDREMADAFHLAGFEVWDVTMQDLCSGAIGLDTFRGVAFVGGFSYADVLGSAKGWAAAVTFHPRAGAELRRFRKRPDTFSLGVCNGCQLLALLGWVGGDPNEDAAEMGPDSQPARPGLLLRHNLSGRYESRWASVRVGPGPALMLRGMEGAVLPVWSAHGEGYVAFSSPELQAQIEARGLAPLHWADDDGNPTEQYPLNPNGSPGGVAGICSCDGRHLAVMPHPERAVRPWQWAWRPPPFDTLTTSPWLQLSINARNWTLEGSC Predict reactive species Full Name: phosphoribosylformylglycinamidine synthase Calculated Molecular Weight: 1338 aa, 145 kDa Observed Molecular Weight: 140-150 kDa GenBank Accession Number: BC167158 Gene Symbol: PFAS Gene ID (NCBI): 5198 RRID: AB_3670793 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O15067 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.