Proteintech, 83111-4-RR, ZO-2 Recombinant monoclonal antibody

Size: 20ul / 100ul The ZO-2 (83111-4-RR) by Proteintech is a Recombinant antibody targeting ZO-2 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, rat samples 83111-4-RR targets ZO-2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HEK-293 cells, C6 cells, HepG2 cells, MCF-7 cells Positive IF/ICC detected in: MCF-7 cells Positive FC (Intra) detected in: A431 cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Zona occludens protein 2 (ZO-2), also known as tight junction protein 2 (TJP2), is a membrane-associated guanylate kinase acting at intercellular contacts of epithelial and endothelial cells but also in their nuclei. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag13534 Product name: Recombinant human TJP2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-289 aa of BC027592 Sequence: MPVRGDRGFPPRRELSGWLRAPGMEELIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVLPGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKVAAIVVKRPRKVQVAALQASPPLDQDDRAFEVMDEFDGRSFRSGYSERSRLNSHGGRSRSWEDSPERGRPHERARSRERDLSRDRSRGRSLERGLDQDHARTRDRSRGRSLERGLDHDFGPSRDRDRDRSRGRSIDQDYERAYHRAYDPDYERAYSPEYRRGARHDARSRGPRSRSREH Predict reactive species Full Name: tight junction protein 2 (zona occludens 2) Calculated Molecular Weight: 134 kDa Observed Molecular Weight: 150 kDa GenBank Accession Number: BC027592 Gene Symbol: TJP2 Gene ID (NCBI): 9414 RRID: AB_3670823 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9UDY2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.