Proteintech, 83152-5-RR, B7-H3/CD276 Recombinant monoclonal antibody

Size: 20ul / 100ul The B7-H3/CD276 (83152-5-RR) by Proteintech is a Recombinant antibody targeting B7-H3/CD276 in WB, ELISA applications with reactivity to Human samples 83152-5-RR targets B7-H3/CD276 in WB, ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: U2OS cells, HEK-293 cells, HeLa cells, LNCaP cells, human placenta tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063). Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: CD276 molecule Calculated Molecular Weight: 57 kDa Observed Molecular Weight: 95-100 kDa GenBank Accession Number: NM_001024736 Gene Symbol: CD276 Gene ID (NCBI): 80381 RRID: AB_3670847 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q5ZPR3 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.