Proteintech, 83191-5-RR, ATP1A1 Recombinant monoclonal antibody

Size: 20ul / 100ul The ATP1A1 (83191-5-RR) by Proteintech is a Recombinant antibody targeting ATP1A1 in IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83191-5-RR targets ATP1A1 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: A431 cells Positive FC (Intra) detected in: HEK-293 cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Background Information ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag5763 Product name: Recombinant human ATP1A1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 347-681 aa of BC050359 Sequence: TAKRMARKNCLVKNLEAVETLGSTSTICSDKTGTLTQNRMTVAHMWFDNQIHEADTTENQSGVSFDKTSATWLALSRIAGLCNRAVFQANQENLPILKRAVAGDASESALLKCIELCCGSVKEMRERYAKIVEIPFNSTNKYQLSIHKNPNTSEPQHLLVMKGAPERILDRCSSILLHGKEQPLDEELKDAFQNAYLELGGLGERVLGFCHLFLPDEQFPEGFQFDTDDVNFPIDNLCFVGLISMIDPPRAAVPDAVGKCRSAGIKVIMVTGDHPITAKAIAKGVGIISEGNETVEDIAARLNIPVSQVNPRDAKACVVHGSDLKDMTSEQLDDI Predict reactive species Full Name: ATPase, Na+/K+ transporting, alpha 1 polypeptide Calculated Molecular Weight: 113 kDa GenBank Accession Number: BC050359 Gene Symbol: ATP1A1 Gene ID (NCBI): 476 ENSEMBL Gene ID: ENSG00000163399 RRID: AB_3670880 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P05023 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.