Proteintech, 83201-3-RR, ATP6 Recombinant monoclonal antibody

Size: 20ul / 100ul The ATP6 (83201-3-RR) by Proteintech is a Recombinant antibody targeting ATP6 in IF/ICC, IF-P, FC (Intra), ELISA applications with reactivity to human samples 83201-3-RR targets ATP6 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IF-P detected in: rat brain tissue Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: U2OS cells Recommended dilution Immunofluorescence (IF)-P: IF-P : 1:175-1:700 Immunofluorescence (IF)/ICC: IF/ICC : 1:150-1:600 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information ATP synthase, also known as FoF1 complex, is a critical mitochondrial OXPHOS enzyme involved in the regulation of mitochondrial ATP production and in the maintenance of the mitochondrial membrane potential. It is composed of three components (F1, Fo and the peripheral stalk). F1, the soluble catalytic core, is above the membrane, inside the matrix of the mitochondria; consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon); Fo, comprising the proton channel, is within the membrane; Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). ATP6 is the a subunit of Fo region. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ATP synthase 6; ATPase subunit 6 Calculated Molecular Weight: 25 kDa GenBank Accession Number: J01415 Gene Symbol: ATP6 Gene ID (NCBI): 4508 RRID: AB_3670889 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P00846 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.