Proteintech, 83223-4-RR, Nectin-4/PVRL4 Recombinant monoclonal antibody

Size: 20ul / 100ul The Nectin-4/PVRL4 (83223-4-RR) by Proteintech is a Recombinant antibody targeting Nectin-4/PVRL4 in IF/ICC, FC, ELISA applications with reactivity to human samples 83223-4-RR targets Nectin-4/PVRL4 in IF/ICC, FC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IF/ICC detected in: MCF-7 cells Positive FC detected in: MCF-7 cells Recommended dilution Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Nectin-4, or PVRL4, belongs to the family of cell adhesion molecules that regulate the formation of adherence functions in epithelial cells through the AF-6 afadin scaffold molecule. Nectin-4 is reported as a histological and serological marker for breast carcinoma. Nectin-4 can be detected as a soluble form of 43 kDa and a transmembrane form of 66 kDa (PMID: 15784625). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Affinity: K D =2.28 x 10 -10 M K Off =2.98 x 10 -5 M K On =2.62 x 10 5 M Immunogen: Recombinant protein Predict reactive species Full Name: poliovirus receptor-related 4 Calculated Molecular Weight: 510 aa, 55 kDa GenBank Accession Number: BC010423 Gene Symbol: Nectin-4 Gene ID (NCBI): 81607 RRID: AB_3670907 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96NY8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.