Proteintech, 83300-2-RR, CARHSP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CARHSP1 (83300-2-RR) by Proteintech is a Recombinant antibody targeting CARHSP1 in IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83300-2-RR targets CARHSP1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: mouse testis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information CARHSP1(calcium-regulated heat-stable protein 1, a 24 kDa protein also known as CRHSP-24) is a ubiquitously expressed phosphoprotein that is comprised of 147 amino acids with nearly 14% proline (PMID: 21990353,30257675). CARHSP1 was discovered as a biomarker for diabetic retinopathy and as a regulator of TNF-α mRNA stability in macrophages (PMID: 21078874). CARHSP1 may be involved in oxidative stress via traffic between stress granules and processing bodies (PMID: 21177848). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2265 Product name: Recombinant human CARHSP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-147 aa of BC003366 Sequence: MSSEPPPPPQPPTHQASVGLLDTPRSRERSPSPLRGNVVPSPLPTRRTRTFSATVRASQGPVYKGVCKCFCRSKGHGFITPADGGPDIFLHISDVEGEYVPVEGDEVTYKMCSIPPKNEKLQAVEVVITHLAPGTKHETWSGHVISS Predict reactive species Full Name: calcium regulated heat stable protein 1, 24kDa Calculated Molecular Weight: 147 aa, 16 kDa GenBank Accession Number: BC003366 Gene Symbol: CARHSP1 Gene ID (NCBI): 23589 RRID: AB_3670969 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9Y2V2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.