Proteintech, 83340-3-RR, CRIP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The CRIP1 (83340-3-RR) by Proteintech is a Recombinant antibody targeting CRIP1 in WB, IHC, ELISA applications with reactivity to human, mouse samples 83340-3-RR targets CRIP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: T-47D cells, HeLa cells, mouse colon tissue Positive IHC detected in: human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Background Information CRIP1 also known as CRIP, CRP1, belongs to the LIM/double zinc finger protein family. CRIP1 has a unique double zinc finger motif, which is mainly expressed in the intestine, and may be involved in intestinal zinc transport (PMID: 31312368, 1946385). CRIP1 is overexpressed in immune cells of the epithelium and may play an important role in gut immunity(PMID: 27836662). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag7588 Product name: Recombinant human CRIP1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-77 aa of BC002738 Sequence: MPKCPKCNKEVYFAERVTSLGKDWHRPCLKCEKCGKTLTSGGHAEHEGKPYCNHPCYAAMFGPKGFGRGGAESHTFK Predict reactive species Full Name: cysteine-rich protein 1 (intestinal) Calculated Molecular Weight: 9 kDa Observed Molecular Weight: 8-9 kDa GenBank Accession Number: BC002738 Gene Symbol: CRIP1 Gene ID (NCBI): 1396 RRID: AB_3671000 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P50238 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.