Proteintech, 83375-2-RR, ICOSLG/B7-H2/CD275 Recombinant monoclonal antibody

Size: 20ul / 100ul The ICOSLG/B7-H2/CD275 (83375-2-RR) by Proteintech is a Recombinant antibody targeting ICOSLG/B7-H2/CD275 in WB, ELISA applications with reactivity to Mouse samples 83375-2-RR targets ICOSLG/B7-H2/CD275 in WB, ELISA applications and shows reactivity with Mouse samples. Tested Applications Positive WB detected in: mouse kidney tissue, mouse brain tissue, mouse spleen tissue, mouse heart tissue Recommended dilution Western Blot (WB): WB : 1:500-1:1000 Background Information ICOSLG, also known as B7H2 or GL50, is a type I transmembrane glycoprotein belonging to the B7 ligand family. ICOSLG is extensively expressed on professional antigen-presenting cells including B cells, macrophages, and dendritic cells, as well as non-lymphoid cells including mesenchymal stem cells, endothelial cells, fibroblasts, and tumor cells (PMID: 33756276; 35800767). It is a specific ligand for the T-cell-specific cell surface receptor ICOS and acts as a co-stimulatory molecule (PMID: 11023515; 11007762). The interaction of ICOSLG and ICOS plays important roles in the activation, proliferation, differentiation, and cytokine production of T cells as well as in the antibody secretion from B cells during secondary immune responses (PMID: 21851236). Specification Tested Reactivity: Mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: icos ligand Calculated Molecular Weight: 36 kDa Observed Molecular Weight: 45-60 kDa GenBank Accession Number: NM_015790.3 Gene Symbol: CD275 Gene ID (NCBI): 50723 RRID: AB_3671027 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9JHJ8-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.