Proteintech, 83386-5-RR, DSC3 Recombinant monoclonal antibody

Size: 20ul / 100ul The DSC3 (83386-5-RR) by Proteintech is a Recombinant antibody targeting DSC3 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 83386-5-RR targets DSC3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: unboiled A431 cells, A431 cells, HaCaT cells Positive IHC detected in: mouse skin tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Desmocollin-3 (DSC3), also known as CDHF3 and DSC4, is a member of the cadherin superfamily of calcium-dependent cell adhesion molecules and a major component of desmosomes. It has 2 isoforms with molecular masses of 100 and 93 kDa. It is particularly expressed in the vagina, testis, skin, oral mucosa and esophagus. Its subcellular localization is at the cell membrane. Post-translational modifications, including glycosylation, have been described. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34648 Product name: Recombinant human DSC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 500-690 aa of NM_001941 Sequence: NRNGNGLRYKKLHDPKGWITIDEISGSIITSKILDREVETPKNELYNITVLAIDKDDRSCTGTLAVNIEDVNDNPPEILQEYVVICKPKMGYTDILAVDPDEPVHGAPFYFSLPNTSPEISRLWSLTKVNDTAARLSYQKNAGFQEYTIPITVKDRAGQAATKLLRVNLCECTHPTQCRATSRSTGVILGK Predict reactive species Full Name: desmocollin 3 Calculated Molecular Weight: 100 kDa Observed Molecular Weight: 90-110 kDa GenBank Accession Number: NM_001941 Gene Symbol: DSC3 Gene ID (NCBI): 1825 RRID: AB_3671042 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q14574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.