Proteintech, 83481-5-RR, RPP30 Recombinant monoclonal antibody

Size: 20ul / 100ul The RPP30 (83481-5-RR) by Proteintech is a Recombinant antibody targeting RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83481-5-RR targets RPP30 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HEK-293 cells, NIH/3T3 cells, U-251 cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: U-251 cells Positive FC (Intra) detected in: U-251 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:750-1:3000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RPP30 is a part of multimeric ribonuclease P complex and ribonuclease MRP complex. It is also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag8600 Product name: Recombinant human RPP30 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-268 aa of BC006991 Sequence: MAVFADLDLRAGSDLKALRGLVETAAHLGYSVVAINHIVDFKEKKQEIEKPVAVSELFTTLPIVQGKSRPIKILTRLTIIVSDPSHCNVLRATSSRARLYDVVAVFPKTEKLFHIACTHLDVDLVCITVTEKLPFYFKRPPINVAIDRGLAFELVYSPAIKDSTMRRYTISSALNLMQICKGKNVIISSAAERPLEIRGPYDVANLGLLFGLSESDAKAAVSTNCRAALLHGETRKTAFGIISTVKKPRPSEGDEDCLPASKKAKCEG Predict reactive species Full Name: ribonuclease P/MRP 30kDa subunit Calculated Molecular Weight: 268 aa, 29 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC006991 Gene Symbol: RPP30 Gene ID (NCBI): 10556 RRID: AB_3671109 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P78346 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.