Proteintech, 83499-3-RR, UPK3A Recombinant monoclonal antibody

Size: 20ul / 100ul The UPK3A (83499-3-RR) by Proteintech is a Recombinant antibody targeting UPK3A in WB, ELISA applications with reactivity to human samples 83499-3-RR targets UPK3A in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, MDA-MB-231 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information Uroplakins are a group of urothelial differentiation-related membrane proteins. They are components of the asymmetric unit membrane (AUM), which forms the apical plaques of mammalian urothelium and is believed to play a role in strengthening the urothelial apical surface thus preventing the cells from rupturing during bladder distention (PMID: 8175808). Uroplakin III (UPK3A) is a promising new urine marker for bladder cancer detection (PMID: 20346489). Loss of UPK3A expression is associated with clinicopathological features of invasive bladder cancer and poor prognosis in upper tract urothelial carcinoma (PMID: 18313120; PMID: 16686732). UPK3A has a calculated molecular weight of 30.7 kDa, with an apparent molecular weight of 47 kDa due to three potential N-glycosylation sites. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: uroplakin 3A Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 47 kDa GenBank Accession Number: NM_006953 Gene Symbol: UPK3A Gene ID (NCBI): 7380 RRID: AB_3671125 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O75631 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.