Proteintech, 83517-3-RR, RPS11 Recombinant monoclonal antibody

Size: 20ul / 100ul The RPS11 (83517-3-RR) by Proteintech is a Recombinant antibody targeting RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83517-3-RR targets RPS11 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: ROS1728 cells, mouse testis tissue, rat testis tissue Positive IHC detected in: mouse testis tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information RPS11 is a ribosomal protein involved in ribosome biogenesis. It is a component of the small ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag34848 Product name: Recombinant human RPS11 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-76 aa of BC007945 Sequence: MADIQTERAYQKQPTIFQNKKRVLLGETGKEKLPRYYKNIGLGFKTPKEAIEGTYIDKKCPFTGNVSIRGRILSGV Predict reactive species Full Name: ribosomal protein S11 Calculated Molecular Weight: 158 aa, 18 kDa Observed Molecular Weight: 18 kDa GenBank Accession Number: BC007945 Gene Symbol: RPS11 Gene ID (NCBI): 6205 RRID: AB_3671142 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P62280 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.