Proteintech, 83521-2-RR, PSMD1 Recombinant monoclonal antibody

Size: 20ul / 100ul The PSMD1 (83521-2-RR) by Proteintech is a Recombinant antibody targeting PSMD1 in WB, IHC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83521-2-RR targets PSMD1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Jurkat cells, A431 cells, SKOV-3 cells, MCF-7 cells, mouse brain tissue, mouse testis tissue, rat brain tissue Positive IHC detected in: human intrahepatic cholangiocarcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PSMD1 is a component of the 26S proteasome and contributes to its function regulation. PSMD1 performs an important role in the regulation of numerous cellular processes through the degradation of proteins targeted for breakdown through ubiquitin. PSMD1 has been found to be closely related to a variety of cancers such as lung adenocarcinoma and breast cancer. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag12009 Product name: Recombinant human PSMD1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 606-953 aa of BC094720 Sequence: AVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD Predict reactive species Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 1 Calculated Molecular Weight: 953 aa, 106 kDa Observed Molecular Weight: 106 kDa GenBank Accession Number: BC094720 Gene Symbol: PSMD1 Gene ID (NCBI): 5707 RRID: AB_3671147 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q99460 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.