Proteintech, 83549-7-RR, Thrombopoietin Recombinant monoclonal antibody

Size: 20ul / 100ul The Thrombopoietin (83549-7-RR) by Proteintech is a Recombinant antibody targeting Thrombopoietin in WB, IHC, IF-P, ELISA applications with reactivity to human, mouse, rat samples 83549-7-RR targets Thrombopoietin in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse liver tissue, HepG2 cells, HuH-7 cells, L02 cells, rat liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse liver tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information Thrombopoietin (TPO) is a primary regulator of megakaryocyte development and platelet production in mammals. Human TPO constitutively circulates and maintains thrombopoiesis through interaction with its cognate receptor, myeloproliferative leukemia protein(MPL). TPO also plays an important role in the maintenance and regulation of hematopoietic stem cells (HSCs). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg0678 Product name: Recombinant mouse Thrombopoietin protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 22-356 aa of NM_001173505.1 Sequence: SPVAPACDPRLLNKLLRDSHLLHSRLSQCPDVDPLSIPVLLPAVDFSLGEWKTQTEQSKAQDILGAVSLLLEGVMAARGQLEPSCLSSLLGQLSGQVRLLLGALQGLLGTQLPLQGRTTAHKDPNALFLSLQQLLRGKVRFLLLVEGPTLCVRRTLPTTAVPSSTSQLLTLNKFPNRTSGLLETNFSVTARTAGPGLLSRLQGFRVKITPGQLNQTSRSPVQISGYLNRTHGPVNGTHGLFAGTSLQTLEASDISPGAFNKGSLAFNLQGGLPPSPSLAPDGHTPFPPSPALPTTHGSPPQLHPLFPDPSTTMPNSTAPHPVTMYPHPRNLSQET Predict reactive species Full Name: thrombopoietin Calculated Molecular Weight: 38KD Observed Molecular Weight: 38 kDa GenBank Accession Number: NM_001173505.1 Gene Symbol: Thrombopoietin Gene ID (NCBI): 21832 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P40226 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.