Proteintech, 83566-1-RR, CORIN Recombinant monoclonal antibody

Size: 20ul / 100ul The CORIN (83566-1-RR) by Proteintech is a Recombinant antibody targeting CORIN in WB, IHC, ELISA applications with reactivity to human, mouse, rat samples 83566-1-RR targets CORIN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: rat heart tissue Positive IHC detected in: mouse heart tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information CORIN is a transmembrane type II serine protease expressed in cardiomyocytes that converts pro-atrial natriuretic peptide into atrial natriuretic peptide, a cardiac hormone that regulates blood pressure. CORIN can be shed into the circulation through autocleavage and metalloproteinase-mediated hydrolysis processes of the membrane form of the protein. Three different soluble fragments derived from corin molecules, with molecular masses of ~180, ~160 and ~100 kDa were described in literature (PMID: 21288900, 29387201). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag15898 Product name: Recombinant human CORIN protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 551-900 aa of BC110451 Sequence: WPEDTDCSQFPEENSDNQTCLMPDEYVEECSPSHFKCRSGQCVLASRRCDGQADCDDDSDEENCGCKERDLWECPSNKQCLKHTVICDGFPDCPDYMDEKNCSFCQDDELECANHACVSRDLWCDGEADCSDSSDEWDCVTLSINVNSSSFLMVHRAATEHHVCADGWQEILSQLACKQMGLGEPSVTKLIQEQEKEPRWLTLHSNWESLNGTTLHELLVNGQSCESRSKISLLCTKQDCGRRPAARMNKRILGGRTSRPGRWPWQCSLQSEPSGHICGCVLIAKKWVLTVAHCFEGRENAAVWKVVLGINNLDHPSVFMQTRFVKTIILHPRYSRAVVDYDISIVELSE Predict reactive species Full Name: corin, serine peptidase Calculated Molecular Weight: 1042 aa, 117 kDa Observed Molecular Weight: 180 kDa GenBank Accession Number: BC110451 Gene Symbol: CORIN Gene ID (NCBI): 10699 RRID: AB_3671181 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9Y5Q5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.