Proteintech, 83590-6-RR, INTS4 Recombinant monoclonal antibody

Size: 20ul / 100ul The INTS4 (83590-6-RR) by Proteintech is a Recombinant antibody targeting INTS4 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 83590-6-RR targets INTS4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, Jurkat cells, HeLa cells, NIH/3T3 cells, mouse testis tissue Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells, U2OS cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information Integrator complex subunit 4 (INTS4), a helical repeat protein, plays a key role in stabilizing nuclease domains and other components. INTS4 is a specific and conserved interaction partner of INTS9/11 heterodimer that does not interact with either subunit individually (PMID: 29471365). INTS4 plays a role in achieving a compact nuclease/pseudonuclease configuration resembling that observed for CPSF73/100 in the histone-processing machinery (PMID: 33548203). Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag9116 Product name: Recombinant human INTS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-331 aa of BC009995 Sequence: MAAHLKKRVYEEFTKVVQPQEEIATKKLRLTKPSKSAALHIDLCKATSPADALQYLLQFARKPVEAESVEGVVRILLEHYYKENDPSVRLKIASLLGLLSKTAGFSPDCIMDDAINILQNEKSHQVLAQLLDTLLAIGTKLPENQAIQMRLVDVACKHLTDTSHGVRNKCLQLLGNLGSLEKSVTKDAEGLAARDVQKIIGDYFSDQDPRVRTAAIKAMLQLHERGLKLHQTIYNQACKLLSDDYEQVRSAAVQLIWVVSQLYPESIVPIPSSNEEIRLVDDAFGKICHMVSDGSWVVRVQAAKLLGSMEQVSSHFLEQTLDKKLMSDLRR Predict reactive species Full Name: integrator complex subunit 4 Calculated Molecular Weight: 963aa,108 kDa; 505aa,57 kDa Observed Molecular Weight: 108 kDa GenBank Accession Number: BC009995 Gene Symbol: INTS4 Gene ID (NCBI): 92105 RRID: AB_3671205 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q96HW7 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.