Proteintech, 83618-3-RR, Presenilin-1-Specific Recombinant monoclonal antibody

Size: 20ul / 100ul The Presenilin-1-Specific (83618-3-RR) by Proteintech is a Recombinant antibody targeting Presenilin-1-Specific in WB, IHC, IF-P, ELISA applications with reactivity to human, rat samples 83618-3-RR targets Presenilin-1-Specific in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat samples. Tested Applications Positive WB detected in: HeLa cells, Caco-2 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: rat brain tissue Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)-P: IF-P : 1:200-1:800 Background Information PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a D30-kDa N-terminal fragment (NTF) and a D20-kDa C-terminal fragment (CTF) by the 26S proteasome (PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody is specific to isoform1(53 kDa), isoform2(52 kDa), isoform4(21 kDa), isoform7(49 kDa) of this protein. Specification Tested Reactivity: human, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: presenilin 1 Calculated Molecular Weight: 53 kDa Observed Molecular Weight: 53 kda GenBank Accession Number: NM_000021 Gene Symbol: PSEN1 Gene ID (NCBI): 5663 RRID: AB_3671231 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P49768 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.