Proteintech, 83644-1-RR, IL-1 alpha Recombinant monoclonal antibody

Size: 20ul / 100ul The IL-1 alpha (83644-1-RR) by Proteintech is a Recombinant antibody targeting IL-1 alpha in WB, IF/ICC, ELISA applications with reactivity to mouse samples 83644-1-RR targets IL-1 alpha in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: RAW 264.7 cells Positive IF/ICC detected in: LPS treated RAW 264.7 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information Interleukin-1α (IL-1 Alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages. Specification Tested Reactivity: mouse Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interleukin 1 alpha Calculated Molecular Weight: 31kd Observed Molecular Weight: 31 kDa GenBank Accession Number: NM_010554 Gene Symbol: Il1a Gene ID (NCBI): 16175 RRID: AB_3671254 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01582 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.