Proteintech, 83647-3-RR, PIP4K2B Recombinant monoclonal antibody

Size: 20ul / 100ul The PIP4K2B (83647-3-RR) by Proteintech is a Recombinant antibody targeting PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications with reactivity to human, mouse samples 83647-3-RR targets PIP4K2B in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, HeLa cells, A549 cells, PC-12 cells, NIH/3T3 cells, C2C12 cells, mouse brain tissue Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: mouse brain tissue Positive FC (Intra) detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PIP4K2B, also named as PIP5K2B, participates in the biosynthesis of phosphatidylinositol-4,5-bisphosphate. PIP4K2B has a homodimer. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4099 Product name: Recombinant human PIP4K2B protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-281 aa of BC027459 Sequence: MSSNCTSTTAVAVAPLSASKTKTKKKHFVCQKVKLFRASEPILSVLMWGVNHTINELSNVPVPVMLMPDDFKAYSKIKVDNHLFNKENLPSRFKFKEYCPMVFRNLRERFGIDDQDYQNSVTRSAPINSDSQGRCGTRFLTTYDRRFVIKTVSSEDVAEMHNILKKYHQFIVECHGNTLLPQFLGMYRLTVDGVETYMVVTRNVFSHRLTVHRKYDLKGSTVAREASDKEKAKDLPTFKDNDFLNEGQKLHVGEESKKNFLEKLKRDVEEILVLSPGRRIA Predict reactive species Full Name: phosphatidylinositol-5-phosphate 4-kinase, type II, beta Calculated Molecular Weight: 416 aa, 47 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC027459 Gene Symbol: PIP4K2B Gene ID (NCBI): 8396 RRID: AB_3671256 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P78356 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.