Proteintech, 83653-5-RR, CEA/CD66e Recombinant monoclonal antibody

Size: 20ul / 100ul The CEA/CD66e (83653-5-RR) by Proteintech is a Recombinant antibody targeting CEA/CD66e in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 83653-5-RR targets CEA/CD66e in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MCF-7 cells, human saliva Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information Carcinoembryonic antigen (CEA), also known as CEACAM5 or CD66e, is a cell surface glycoprotein belonging to the immunoglobulin superfamily, mainly serving as a cell adhesion molecule mediating intercellular contact by both homophilic and heterophilic binding (PMID: 21731662). CEA inhibits anoikis and plays a role in tumorigenesis and metastasis. CEA has been found to be overexpressed in a wide variety of human cancers, including colon, breast, and lung (PMID: 17167768). CEA is a tumor marker and is routinely exploited for diagnosis. This antibody is raised against 35-685 aa of human CEA/CD66e and can cross-react with CEACAM1/CD66a and CEACAM3/CD66d. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: carcinoembryonic antigen-related cell adhesion molecule 5 Calculated Molecular Weight: 77 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: NM_004363 Gene Symbol: CEA Gene ID (NCBI): 1048 RRID: AB_3671261 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P06731-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.