Proteintech, 83663-2-RR, PHKG2 Recombinant monoclonal antibody

Size: 20ul / 100ul The PHKG2 (83663-2-RR) by Proteintech is a Recombinant antibody targeting PHKG2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse samples 83663-2-RR targets PHKG2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HepG2 cells, MCF-7 cells, mouse liver tissue Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HEK-293 cells Positive FC (Intra) detected in: HEK-293T cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PHKG2 (phosphorylase kinase catalytic subunit gamma 2), it is expected to be located in the cytoplasm, which is enriched in the testis. Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. It may regulates glycogeneolysis in the testis. Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The gamma subunit also includes the skeletal muscle and hepatic isoforms, and the hepatic isoform is encoded by this gene. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. The molecular weight of PHKG2 is 46 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6829 Product name: Recombinant human PHKG2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-298 aa of BC002541 Sequence: MTLDVGPEDELPDWAAAKEFYQKYDPKDVIGRGVSSVVRRCVHRATGHEFAVKIMEVTAERLSPEQLEEVREATRRETHILRQVAGHPHIITLIDSYESSSFMFLVFDLMRKGELFDYLTEKVALSEKETRSIMRSLLEAVSFLHANNIVHRDLKPENILLDDNMQIRLSDFGFSCHLEPGEKLRELCGTPGYLAPEILKCSMDETHPGYGKEVDLWACGVILFTLLAGSPPFWHRRQILMLRMIMEGQYQFSSPEWDDRSSTVKDLISRLLQVDPEARLTAEQALQHPFFERCEGSQ Predict reactive species Full Name: phosphorylase kinase, gamma 2 (testis) Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC002541 Gene Symbol: PHKG2 Gene ID (NCBI): 5261 RRID: AB_3671270 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P15735 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.