Proteintech, 83682-4-RR, CDCA7L Recombinant monoclonal antibody

Size: 20ul / 100ul The CDCA7L (83682-4-RR) by Proteintech is a Recombinant antibody targeting CDCA7L in WB, FC (Intra), ELISA applications with reactivity to human samples 83682-4-RR targets CDCA7L in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: A549 cells, HEK-293T cells, HeLa cells, K-562 cells, MCF-7 cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Cell division cycle-associated 7-like protein (CDCA7L) is also known as HR1 and JPO2 and belongs to the JPO protein family. CDCA7L is identified as a target gene of c-Myc and is frequently dysregulated in multiple cancers. CDCA7L can complement the c-Myc transformation-defective mutant W135E and potentiate Myc-mediated transformation (PMID: 24141559, PMID: 37140023). CDCA7L translocates from cytoplasm to nucleus under dexamethasone induction. CDCA7L is widely expressed. CDCA7L has 4 isoforms produced by alternative splicing (refer to UniProt). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag10944 Product name: Recombinant human CDCA7L protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-269 aa of BC009352 Sequence: MELATRYQIPKEVADIFNAPSDDEEFVGFRDDVPMETLSSEESCDSFDSLESGKQQDVRFHSKYFTEELRRIFIEDTDSETEDFAGFTQSDLNGKTNPEVMVVESDLSDDGKASLVSEEEEDEEEDKATPRRSRSRRSSIGLRVAFQFPTKKLANKPDKNSSSEQLFSSARLQNEKKTILERKKDCRQVIQREDSTSESEDDSRDESQESSDALLKRTMNIKENKAMLAQLLAELNSMPDFFPVRTPTSASRKKTVRRAFSEGQITRRM Predict reactive species Full Name: cell division cycle associated 7-like Calculated Molecular Weight: 454 aa, 52 kDa Observed Molecular Weight: 52 kDa GenBank Accession Number: BC009352 Gene Symbol: CDCA7L Gene ID (NCBI): 55536 RRID: AB_3671283 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q96GN5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.