Proteintech, 83689-4-RR, HSP47/SERPINH1 Recombinant monoclonal antibody

Size: 20ul / 100ul The HSP47/SERPINH1 (83689-4-RR) by Proteintech is a Recombinant antibody targeting HSP47/SERPINH1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse samples 83689-4-RR targets HSP47/SERPINH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: A2780 cells, HeLa cells, A549 cells, HT-1080 cells, MCF-7 cells, HepG2 cells, NIH/3T3 cells Positive IHC detected in: human ovarian cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1201 Product name: Recombinant Human HSP47/SERPINH1 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-418 aa of NM_001235.5 Sequence: AEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL Predict reactive species Full Name: serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) Calculated Molecular Weight: 46kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: NM_001235.5 Gene Symbol: HSP47/SERPINH1 Gene ID (NCBI): 871 RRID: AB_3671292 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P50454 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.