Proteintech, 83753-4-RR, Decorin Recombinant monoclonal antibody

Size: 20ul / 100ul The Decorin (83753-4-RR) by Proteintech is a Recombinant antibody targeting Decorin in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83753-4-RR targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: MCF-7 cells, ROS1728 cells, mouse colon tissue Positive FC (Intra) detected in: HEK-293 cells, HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6275 Product name: Recombinant human DCN protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-359 aa of BC005322 Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK Predict reactive species Full Name: decorin Calculated Molecular Weight: 359 aa, 40 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC005322 Gene Symbol: Decorin Gene ID (NCBI): 1634 RRID: AB_3671350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P07585 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.