Proteintech, 83763-5-RR, Granzyme B Recombinant monoclonal antibody

Size: 20ul / 100ul The Granzyme B (83763-5-RR) by Proteintech is a Recombinant antibody targeting Granzyme B in WB, ELISA applications with reactivity to human samples 83763-5-RR targets Granzyme B in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: NK-92 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information GZMB(Granzyme B) is also named as CGL1, CSPB, CTLA1, GRB and belongs to the Granzyme subfamily. This enzyme is necessary for target cell lysis in cell-mediated immune responses. The cytotoxic lymphocyte protease granzyme B (GzmB) can promote apoptosis through direct processing and activation of members of the caspase family. GzmB can also cleave the BH3-only protein, BID, to promote caspase-independent mitochondrial permeabilization (PMID:17283187). GzmB induces laminB degradation in isolated nuclei less efficiently than GzmA (PMID:11331782). This full length protein has 2 glycosylation sites and a signal peptide. Unglycosylated human granzyme B is 26 kDa and high mannose glycosylated is 32 kDa and only 32kDa or smaller forms of granzyme B are accumulated within nuclei (PMID:8626751). GzmB also forms dimers. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) Calculated Molecular Weight: 247 aa, 28 kDa Observed Molecular Weight: 33 kDa GenBank Accession Number: BC030195 Gene Symbol: Granzyme B Gene ID (NCBI): 3002 RRID: AB_3671358 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P10144 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.