Proteintech, 83840-7-RR, Phospho-PKC Alpha (Ser657) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PKC Alpha (Ser657) (83840-7-RR) by Proteintech is a Recombinant antibody targeting Phospho-PKC Alpha (Ser657) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 83840-7-RR targets Phospho-PKC Alpha (Ser657) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: Calyculin A treated PC-3 cells, PMA treated NIH/3T3 cells, Calyculin A treated HSC-T6 cells Positive IF/ICC detected in: NIH/3T3 cells Positive FC (Intra) detected in: Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information PKCs are a family of serine/threonine kinases involved in various processes in cells including proliferation, differentiation, cell survival, and apoptosis. PKC family is composed of three different subgroups: conventional (cPKC), the novel (nPKC) and atypical (aPKC). PKCα, β1, β2, and γ belong to cPKC, PKCδ, ε, η and θ are nPKC, whereas aPKC comprises of PKCζ and λ/ι. All PKCs consist of the N-terminal regulatory region and C-terminal catalytic region (kinase domain). PKCs are physiologically activated by various extracellular signals transduced by hormones, growth factors, cytokines or antigens. The presence of activated PKCs on internal membranes leads to the phosphorylation of various interacting proteins. (PMID: 32466765, PMID: 12417016) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein kinase C, alpha Calculated Molecular Weight: 77 kDa Observed Molecular Weight: 77 kDa GenBank Accession Number: AK055431 Gene Symbol: PKC Alpha Gene ID (NCBI): 5578 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P17252 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.