Proteintech, 83896-1-RR, CD25/IL-2RA Recombinant monoclonal antibody

Size: 20ul / 100ul The CD25/IL-2RA (83896-1-RR) by Proteintech is a Recombinant antibody targeting CD25/IL-2RA in WB, ELISA applications with reactivity to human samples 83896-1-RR targets CD25/IL-2RA in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MJ cells Positive IF/ICC detected in: MJ cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information Proliferation of T lymphocytes is triggered by the interaction of IL-2 with its specific receptor following T lymphocyte activation. The receptor for IL-2 has three forms, generated by different combinations of three different proteins, the alpha chain (IL-2R alpha), the beta chain (IL-2R beta), and the gamma chain (IL-2R gamma) (PMID: 8476561). IL-2R alpha (also known as CD25) is a type I transmembrane protein present in activated T cells, activated B cells, some thymocytes, myeloid precursors, and oligodendrocytes. CD25 is also found on natural CD4+ Foxp3+ Treg cells (PMID: 22585674). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: interleukin 2 receptor, alpha Calculated Molecular Weight: 272 aa, 31 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC156627 Gene Symbol: IL2RA Gene ID (NCBI): 3559 RRID: AB_3671477 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P01589 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.