Proteintech, 84128-5-RR, HER2/ErbB2 Recombinant monoclonal antibody

Size: 20ul / 100ul The HER2/ErbB2 (84128-5-RR) by Proteintech is a Recombinant antibody targeting HER2/ErbB2 in WB, IF/ICC, ELISA applications with reactivity to mouse samples 84128-5-RR targets HER2/ErbB2 in WB, IF/ICC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: 4T1 cells Positive IF/ICC detected in: 4T1 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information Her2, or ErbB2, is a member of the EGF receptor family of receptor tyrosine kinases. It has no ligand-binding domain of its own. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Fusion Protein Predict reactive species Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) Calculated Molecular Weight: 139 kDa Observed Molecular Weight: 185 kDa GenBank Accession Number: NM_001003817.1 Gene Symbol: Erbb2 Gene ID (NCBI): 13866 RRID: AB_3671687 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P70424 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.