Proteintech, 84145-2-RR, CDC25A Recombinant monoclonal antibody

Size: 20ul / 100ul The CDC25A (84145-2-RR) by Proteintech is a Recombinant antibody targeting CDC25A in IHC, IF/ICC, ELISA applications with reactivity to human samples 84145-2-RR targets CDC25A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information CDC25A belongs to the MPI phosphatase family. It is a tyrosine protein phosphatase which functions as a dosage-dependent inducer of mitotic progression. CDC25A directly dephosphorylates CDC2 and stimulates its kinase activity. It also dephosphorylates CDK2 in complex with cyclin E, in vitro. CDC25A overexpression in human cancers might contribute to tumorigenesis. This protein can be phosphorylated to reveal the molecular weight from 55 kDa to 70 kDa(PMID:21376736; 8156993).The antibody is specific to CDC25A. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cell division cycle 25 homolog A (S. pombe) Calculated Molecular Weight: 59 kDa GenBank Accession Number: NM_001789 Gene Symbol: CDC25A Gene ID (NCBI): 993 RRID: AB_3671708 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P30304 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.