Proteintech, 84192-4-RR, Ki-67 Recombinant monoclonal antibody

Size: 20ul / 100ul The Ki-67 (84192-4-RR) by Proteintech is a Recombinant antibody targeting Ki-67 in WB, IHC, IF/ICC, IF-P, ELISA applications with reactivity to human samples 84192-4-RR targets Ki-67 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, MCF-7 cells, Ramos cells Positive IHC detected in: human tonsillitis tissue, human skin cancer tissue, Human Spleen tissue, Human renal cancer, human malignant melanoma tissue, human prostate cancer tissue, human Breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human lung cancer tissue, human thyroid cancer tissue Positive IF/ICC detected in: HeLa cells, MCF-7 cells, U2OS cells, HepG2 cells, A431 cells, A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information The Ki-67 protein (also known as MKI67) is a cellular marker for proliferation. Ki67 is present during all active phases of the cell cycle (G1, S, G2 and M), but is absent in resting cells (G0). Cellular content of Ki-67 protein markedly increases during cell progression through S phase of the cell cycle. Therefore, the nuclear expression of Ki67 can be evaluated to assess tumor proliferation by immunohistochemistry. It has been demonstrated to be of prognostic value in breast cancer. In head and neck cancer, several studies have reported an association between high proliferative activity and poorer prognosis. Specification Tested Reactivity: human Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: antigen identified by monoclonal antibody Ki-67 Calculated Molecular Weight: 359 kDa Observed Molecular Weight: 359 kDa GenBank Accession Number: NM_002417 Gene Symbol: KI67 Gene ID (NCBI): 4288 RRID: AB_3671744 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P46013 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.