Proteintech, 84270-5-RR, CD72 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD72 (84270-5-RR) by Proteintech is a Recombinant antibody targeting CD72 in WB, IF/ICC, ELISA applications with reactivity to human samples 84270-5-RR targets CD72 in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Raji cells Positive IF/ICC detected in: Ramos cells, Raji cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information CD72 is a type II transmembrane glycoprotein of 39-45 kDa and is expressed as a disulphide-linked homodimer (PMID: 1711157). CD72 is a pan B-cell marker covering the full range of differentiation from the very early stages of B cells to mature surface Ig+ B cells (PMID: 1384316). It is expressed in all stages of B cell development except plasma cells. CD72 is also expressed by dendritic cells, tissue macrophages in the red pulp of the spleen and von Kupffer cells in the liver (PMID: 8561576; 1384316). CD72 is the ligand for CD5 (PMID: 1711157). CD72 is a negative regulator of BCR signaling (PMID: 9590210). Interaction of CD100 with CD72 strictly tunes the strength of BCR signals and is essential for maintaining immunological homeostasis as well as generating a proper immune response (PMID: 16113236). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: CD72 molecule Calculated Molecular Weight: 359 aa, 40 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC030227 Gene Symbol: CD72 Gene ID (NCBI): 971 RRID: AB_3671816 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P21854 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.