Proteintech, 84316-7-RR, HNF4A Recombinant monoclonal antibody

Size: 20ul / 100ul The HNF4A (84316-7-RR) by Proteintech is a Recombinant antibody targeting HNF4A in IHC, IF/ICC, ELISA applications with reactivity to human samples 84316-7-RR targets HNF4A in IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive IHC detected in: mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information HNF4A (Hepatocyte nuclear factor 4 α) is highly conserved between humans and mice. Using conditional knockouts, epithelial HNF4A is indispensable for protecting against acute chemical-induced colitis and spontaneous colonic inflammation in aged animals (PMID:35792863). Moreover, reduced expression of HNF4A has been reported in liver fibrosis and viral vector-mediated HNF4A restoration has previously been demonstrated to reverse features of chronic liver injury. Therefore, HNF4A has been suggested as a therapeutic target for the treatment of liver fibrosis (PMID:34453962). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag24647 Product name: Recombinant human HNF4A protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC137539 Sequence: MRLSKTLVDMDMADYSAALDPAYTTLEFENVQVLTMGNDTSPSEGTNLNAPNSLGVSALCAICGDRATGKHYGASSCDGCKGFFRRSVRKNHMYSCRFSRQCVVDKDKRNQCRYCRLKKCFRAGMKKEAVQNERDRISTRRSSYEDSSLPSINALLQAEVLSRQITSPVSGINGDIRAKKIASIADVCESMKEQLLVLVEWAKYIPAFCELPLDDQVALLRAHAGEHLLLGATKRSMVFKDVLLLGNDYIVPRHCPELAEMSRVSIRILDELVLPFQELQIDDNEYAYLKAIIFFDPDAKGLSDPGKIKRLRSQVQVSLEDYINDRQYDSRGRFGELLLLLPTLQSITWQ Predict reactive species Full Name: hepatocyte nuclear factor 4, alpha Calculated Molecular Weight: 53 kDa GenBank Accession Number: BC137539 Gene Symbol: HNF4A Gene ID (NCBI): 3172 RRID: AB_3671857 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P41235 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.