Proteintech, 84357-1-RR, POLR2A Recombinant monoclonal antibody

Size: 20ul / 100ul The POLR2A (84357-1-RR) by Proteintech is a Recombinant antibody targeting POLR2A in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse, monkey samples 84357-1-RR targets POLR2A in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, monkey samples. Tested Applications Positive WB detected in: COS-7 cells Positive IHC detected in: mouse pancreas tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing. Specification Tested Reactivity: human, mouse, monkey Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa Calculated Molecular Weight: 217 kDa Observed Molecular Weight: 245 kDa GenBank Accession Number: NM_000937 Gene Symbol: POLR2A Gene ID (NCBI): 5430 RRID: AB_3671895 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P24928 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.