Proteintech, 84403-1-RR, Phospho-IkB Beta (Thr19/Ser23) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-IkB Beta (Thr19/Ser23) (84403-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), ELISA applications with reactivity to human samples 84403-1-RR targets Phospho-IkB Beta (Thr19/Ser23) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: TNF alpha and Calyculin A treated HT-29 cells Positive FC (Intra) detected in: TNF alpha and Calyculin A treated HT-29 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information IkB Beta is also named as NF-kappa-B inhibitor beta (NFKBIB) and TRIP-9. IKBB is one of the major isoforms of the NFKB inhibitor which preferentially interacts with c-Rel or p65 NF-kB dimers (PMID: 26227166). IKBB was reported to be associated with tumor growth inhibition and metastasis in lung cancer (PMID: 11402320). IKBB also inhibits cancer cell regulation through inactivation of the NFkB pathway (PMID: 18950638). Exposure of cells to a variety of extracellular stimuli leads to the rapid phosphorylation, ubiquitination, and ultimately proteolytic degradation of IkB, which frees NF-kB to translocate to the nucleus where it regulates gene transcription (PMID: 10837071). The antibody is phosphorylated at Thr19 and Ser23. Specification Tested Reactivity: human Cited Reactivity: rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, beta Calculated Molecular Weight: 356 aa, 38 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC015528 Gene Symbol: IkB Beta Gene ID (NCBI): 4793 RRID: AB_3671932 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q15653 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.