Proteintech, 84420-7-RR, CD30/TNFRSF8 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD30/TNFRSF8 (84420-7-RR) by Proteintech is a Recombinant antibody targeting CD30/TNFRSF8 in WB, IHC, ELISA applications with reactivity to mouse samples 84420-7-RR targets CD30/TNFRSF8 in WB, IHC, ELISA applications and shows reactivity with mouse samples. Tested Applications Positive WB detected in: EL-4 cells Positive IHC detected in: mouse spleen tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information CD30, also known as TNFRSF8 or Ki-1, is a transmembrane glycoprotein belonging to the tumor necrosis factor receptor superfamily. It is composed of an extracellular domain with six cysteine-rich repeats, a transmembrane segment, and an intracellular domain (PMID: 1310894). CD30 is expressed on a small subset of activated T and B lymphocytes, and a variety of lymphoid neoplasms, with the highest expression in classical Hodgkin's lymphoma and anaplastic large cell lymphoma (PMID: 9826579; 28885612). The CD30 ligand (CD30L or CD153) is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (PMID: 8701986). Signal transduction of CD30/CD30L utilizes signal transducers, TNFR-associated factors (TRAF1, 2, 3 and 5) (PMID: 9826579). Specification Tested Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1307 Product name: Recombinant Mouse CD30/TNFRSF8 protein (His Tag) Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-281 aa of NM_009401.2 Sequence: FPTDRPLKTTCAGDLSHYPGEAARNCCYQCPSGLSPTQPCPRGPAHCRKQCAPDYYVNEDGKCTACVTCLPGLVEKAPCSGNSPRICECQPGMHCCTPAVNSCARCKLHCSGEEVVKSPGTAKKDTICELPSSGSGPNCSNPGDRKTLTSHATPQAMPTLESPANDSARSLLPMRVTNLVQEDATELVKVPESSSSKAREPSPDPGNAEKNMTLELPSPGTLPDISTSENSKEPASTASTLSLVVDAWTSSRMQPTSPLSTGT Predict reactive species Full Name: tumor necrosis factor receptor superfamily, member 8 Calculated Molecular Weight: 53kDa Observed Molecular Weight: 105-120 kDa GenBank Accession Number: NM_009401.2 Gene Symbol: Tnfrsf8 Gene ID (NCBI): 21941 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q60846 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.