Proteintech, 84464-4-RR, LEPR Recombinant monoclonal antibody

Size: 20ul / 100ul The LEPR (84464-4-RR) by Proteintech is a Recombinant antibody targeting LEPR in WB, ELISA applications with reactivity to human, mouse samples 84464-4-RR targets LEPR in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: K-562 cells, HeLa cells, HepG2 cells, L02 cells, mouse brain tissue Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Background Information The hormone leptin, secreted predominantly from adipose tissue, plays a crucial role in the regulation of numerous neuroendocrine functions, from energy homeostasis to reproduction (PMID: 22249808). Leptin acts through the leptin receptor (LEPR, also known as OB-R), a single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, LEPR mediates signaling through JAK2/STAT3 (PMID: 9102398). LEPR plays an important role in the regulation of glucose homeostasis via feeding and adiposity. Alternatively spliced transcript variants encoding different isoforms of LEPR have been described. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant Protein Predict reactive species Full Name: leptin receptor Calculated Molecular Weight: 132 kDa Observed Molecular Weight: 120 kDa GenBank Accession Number: NM_002303 Gene Symbol: LEPR Gene ID (NCBI): 3953 RRID: AB_3671986 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P48357 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.