Proteintech, 84470-4-RR, IGFBP3 Recombinant monoclonal antibody

Size: 20ul / 100ul The IGFBP3 (84470-4-RR) by Proteintech is a Recombinant antibody targeting IGFBP3 in WB, ELISA applications with reactivity to human samples 84470-4-RR targets IGFBP3 in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: SKOV-3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information Insulin-like growth factor binding protein-3 (IGFBP-3) contains a 27 amino acid putative signal sequence followed by a mature protein of 264 amino acids with 18 cysteine residues clustered near the N- and C-terminus. Accordingly, expression of the cloned IGFBP-3 cDNA in mammalian tissue culture cells results in secretion of the protein into the culture medium. IGFBP-3 shares high homology (33% amino acid identity) including conservation of all 18 cysteine residues with a smaller human IGF-binding protein (BP-28) identified in amniotic fluid. IGFBP-3 has one or more glycosylation sites with a protein core of ∼30 kDa. Western blots revealed that the 39-45 kDa IGFBP-3 fragment is a glycoprotein. (PMID: 23403918, PMID: 24926947, PMID: 19887447) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: FusionProtein Predict reactive species Full Name: insulin-like growth factor binding protein 3 Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 32-37 kDa GenBank Accession Number: NM_000598.5 Gene Symbol: IGFBP3 Gene ID (NCBI): 3486 RRID: AB_3671994 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P17936-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.