Proteintech, 84509-2-RR, CD37 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD37 (84509-2-RR) by Proteintech is a Recombinant antibody targeting CD37 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 84509-2-RR targets CD37 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Daudi cells, Raji cells, Ramos cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Daudi cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Background Information CD37 is a membrane protein of the tetraspanin superfamily which are characterized by the presence of four conserved transmembrane regions. Many of these members are expressed on leukocytes and have been implicated in signal transduction, cell-cell interactions, and cellular activation and development. CD37 plays a role in B-cell function, and may also play a role in T-cell-B-cell interactions (PMID: 10891477). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: FusionProtein Predict reactive species Full Name: CD37 molecule Calculated Molecular Weight: 32kDa Observed Molecular Weight: 40-52 kDa GenBank Accession Number: NM_001774.3 Gene Symbol: CD37 Gene ID (NCBI): 951 RRID: AB_3672018 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P11049-1 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.