Proteintech, 84534-5-RR, MDMX/MDM4 Recombinant monoclonal antibody

Size: 20ul / 100ul The MDMX/MDM4 (84534-5-RR) by Proteintech is a Recombinant antibody targeting MDMX/MDM4 in WB, IHC, ELISA applications with reactivity to human samples 84534-5-RR targets MDMX/MDM4 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, HEK-293T cells, A549 cells, MCF-7 cells, U2OS cells Positive IHC detected in: human ovary cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Background Information MDM4 is also named as MDMX. It inhibits the activity of the tumor suppressor p53 by primarily cooperating with the p53 feedback regulator MDM2. MDM4 resides mostly in the cytoplasm, but can be recruited to the nucleus by MDM2.(PMID:16511572). The predicted mass of the protein is 54 kDa, while the observed mass is 70-80 kDa, a difference which stated is probably due to phosphorylation or other posttranslational modification(PMID:9226370, 28097652). It can be ubiquitinated and degraded by MDM2(PMID:16163388). It has 5 isoforms produced by alternative splicing. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag26678 Product name: Recombinant human MDM4 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-332 aa of BC067299 Sequence: MTSFSTSAQCSTSDSACRISPGQINQVRPKLPLLKILHAAGAQGEMFTVKEVMHYLGQYIMVKQLYDQQEQHMVYCGGDLLGELLGRQSFSVKDPSPLYDMLRKNLVTLATATTDAAQTLALAQDHSMDIPSQDQLKQSAEESSTSRKRTTEDDIPTLPTSEHKCIHSREDEDLIENLAQDETSRLDLGFEEWDVAGLPWWFLGNLRSNYTPRSNGSTDLQTNQDVGTAIVSDTTDDLWFLNESVSEQLGVGIKVEAADTEQTSEEVGKVSDKKVIEVGKNDDLEDSKSLSDDTDVEVTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYS Predict reactive species Full Name: Mdm4 p53 binding protein homolog (mouse) Calculated Molecular Weight: 490 aa, 55 kDa Observed Molecular Weight: 70-76 kDa GenBank Accession Number: BC067299 Gene Symbol: MDMX/MDM4 Gene ID (NCBI): 4194 RRID: AB_3672041 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: O15151 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.