Proteintech, 84564-6-RR, Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Estrogen Receptor α (Ser118) (84564-6-RR) by Proteintech is a Recombinant antibody targeting Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications with reactivity to human samples 84564-6-RR targets Phospho-Estrogen Receptor α (Ser118) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: T-47D cells Positive FC (Intra) detected in: λ phosphatase treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: estrogen receptor 1 Calculated Molecular Weight: 595 aa, 66 kDa Observed Molecular Weight: 66 kDa GenBank Accession Number: BC128573 Gene Symbol: ER Gene ID (NCBI): 2099 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P03372 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.