Proteintech, 84590-2-RR, Phospho-CRKL (Tyr207) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-CRKL (Tyr207) (84590-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 84590-2-RR targets Phospho-CRKL (Tyr207) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells Positive FC (Intra) detected in: λ phosphatase treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information CrkL (v-crk avian sarcoma virus CT10 oncogene homolog-like) is a cytoplasmic adaptor protein that belongs to the Crk family of adaptors. Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 39 kDa GenBank Accession Number: BC043500 Gene Symbol: CRKL Gene ID (NCBI): 1399 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P46109 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.